Monday, December 21, 2009

STUDY OF TRANSVERSE SECTIONS-pharm-D labmanual

STUDY OF TRANSVERSE SECTIONS
AIM:
To study the transverse section of Senna, cassia, ephedra, Podophyllum
Principle:
A segment of the body that is at right angles to the body's longitudinal axis.
Senna the sennas, is a large genus of around 250 species of flowering plants in the family Fabaceae, subfamily Caesalpinioideae.
Almost all species were at one time or another placed in Cassia, a close relative which until recent decades served as a "wastebin taxon" to hold all Cassiinae
Ephedra is the dried herbaceous stem of Ephedra sinica Stapf, Ephedra intermedia Schrenk et C. A. Mey. or Ephedra equisetina Bge. (Fam. Ephedraceae)
Action: To induce perspiration for dispelling cold, to relieve asthma, and to cause diuresis
Podophyllum peltatum, Linne (N.O. Berberidece), is a small herb with a long perennial creeping rhizome.
The drug was well known to the North American Indians as an emetic and vermifuge;

Glassware requirement:
a small beaker
forceps
biological leaf cutter/blade
microscopic slide
distilled water

PROCEDURE:
Take the leaf of senna or cassia
wash it carefully with distilled water
take out from the beaker using forceps
using a sterile blade slice out the leaf carefully
obtain thin slice of leaf
mount in on to the microscopic slide carefully
observe it under microscope clearly
repeat the same procedure with the stem of ephedra and podophyllum
observe them clearly under compound microscope for the transverse section of the stem and leaf

OBSERVATION/RESULT
Transverse section of the leaves senna and cassia. as well stems ephedra and podophyllum were observed clearly
STEM:

LEAF

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