Monday, December 21, 2009

plant physiological experiment of photosynthesis-pharm-D labmanual

AIM: To perform the plant physiological experiment of photosynthesis
plant physiology is the study of the function, or physiology, of plants. Fundamental processes such as photosynthesis, respiration, plant nutrition, plant hormone functions, tropisms, nastic movements, photoperiodism, photomorphogenesis, circadian rhythms, environmental stress physiology, seed germination, dormancy and stomata function and transpiration,
PHOTOSYNTHESIS:is a process that converts carbon dioxide into organic compounds, especially sugars, using the energy from sunlight.
2 H2O + 2 NADP+ + 2 ADP + 2 Pi + light → 2 NADPH + 2 H+ + 2 ATP + O2
Respiration is the process by which a carbohydrate is broken down by oxygen to produce energy, carbon dioxide and water. The net chemical reaction is the reverse of the photosynthetic reaction.

pondweed (from a fish tank or a lake)
a clear bowl
a clear jar (smaller in diameter than the bowl)
supports (e.g. cotton reels)
Set up the materials as in the diagram.
Place the whole experiment where it gets enough light to photosynthesise but doesn't get too hot.
Leave it for several hours.
The oxygen should have collected in the upside-down jar.
To test it carefully light a match and then lift the jar quickly, placing the lit match into the jar.
If oxygen is present, the flame should get brighter for a short time.
flame became so bright such that depicting the process of photosynthesis and took place

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